Materials & Methods
Mapping Procedures
The two genes (PDHA1 and ADD1) were chosen as possible candidate genes for meat quality based on their observed physiological functions in other mammalian species. Primers for use in polymerase chain reaction (PCR) amplification were designed based on porcine sequence available for each gene in GenBank. PCR conditions were optimized for each gene separately. Sequencing and comparisons of breed DNA pools were completed in order to search for sequence variation among the breeds. Physical mapping was achieved by use of a pig-rodent somatic cell hybrid panel (Yerle et al., 1996).