A randomized complete block design was utilized in this research. The six replications were assigned as blocks. A one-factor experiment (treatment: nonES and ES) and a two-factor experiment (treatment: nonES and ES; time: day 0, 2, 4 or day 2, 4) were used for comparisons of different measurements during refrigerated storage (Figure 1). A general linear model (GLM) was analyzed by the Statistical Analysis System (SAS Institute, Inc., 1999). If significant two-way interaction occurred, simple main effects were tested by contrast. Multiple comparisons of significant differences were determined by Duncan's multiple range at á = 0.05.
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| Figure 1. Experimental procedure: Effects of electrical stimulation (ES) on lipid oxidation and quality of roast beef. |