C.T. Li*, M. Wick*1 and D.B. Min#
The Ohio State University Department of Animal Sciences*, and
The Ohio State University Department of Food Science and Technology#
Lipid oxidation in fresh lamb tissue (n = 6) and a corn oil control (n = 6) was measured using two peroxide value assays: 1) AOCS peroxide value (PV) method and 2) a new spectrophotometric peroxide method (SPM) in addition to the 2-thiobarbituric acid (TBA) method. The PV determined by the AOCS and SPM methods were 1.4 and 1.5 meq/kg (P < 0.05), respectively. Coefficients of variation (CV) for AOCS and SPM methods were 33.7% and 10.7%, respectively. The TBA mean of fresh lamb tissue was 0.10 mg/g with a CV of 14.5%. The PV of corn oil, determined by the AOCS and SPM methods, were 52.2 and 51.7 μg/kg (P < 0.05), respectively. The CV of AOCS and SPM methods were 0.36% and 0.21%, respectively. The TBA mean was 0.39 mg/g with a CV of 4.6%. Low CV indicate that the SPM method has better reproducibility than either the TBA or AOCS method in measuring lipid oxidation in both lamb tissue and corn oil. In addition, the SPM reports results faster than TBA and AOCS method. These results demonstrate that SPM is more rapid and reproducible than either AOCS or TBA method and could be an effectively method to evaluate lipid oxidation in animal tissue.
1For more information, contact at: 230A Plumb Hall, 2027 Coffey Road, Columbus, OH 43210, (614) 292-7516, Fax: (614) 292-7116, Email: wick.13@osu.edu