The research was divided into two experiments. The first experiment was to determinate the effect on Staphylococcus aureus and Salmonella derby due to treatment with 0, 1, 2, or 3% lactic acid. The second experiment was the same as Experiment 1 but used 0, 20, 40, or 60 ppm of sodium hypochlorite solutions. Both of there experiments added the anti-microbial material to 8 log CFU solution of Staphylococcus aureus or salmonella derby. The mixture was incubated at 0 to 4C. After 0, 1, 2, 3, 4, 5, 24, and 48 hours colonies were counted.
Experiment Design
For Experiments 1 and 2, experimental design for microbial levels was a 4 x 8 factorial using a completely randomized design. The experiments have two main effects.
Factor 1 is concentration: 0, 1, 2, or 3% (v/v) of lactic acid for Experiment 1 and 0, 20, 40, or 60 ppm of sodium hypochlorite in Experiment 2.
Factor 2 in both of experiments were contact time: 0, 1, 2, 3, 4, 5, 24, and 48 hours.
Statistical Analysis
Difference due to microbiological count between treatments were examine for significance by analysis of variance.