Ohio State University Extension Bulletin

Research and Reviews: Dairy 2001

Special Circular 182-01


Conjugated Linoleic Acid in Milk from Cows on Pasture

Materials and Methods

Milk from bulk tanks was sampled from 11 Ohio herds during May 2000. The milk was analyzed for fatty acids using a gas chromatograph and modifications of the procedure described by Sukhija and Palmquist (1988). The characteristics of the herds, milk yield, and feeding practices were provided via a survey to the farmers. The milk composition data were from the Dairy Herd Improvement laboratory (DHI Cooperative, Inc., Columbus, OH). Characteristics of the herds are provided in Table 1. All, except one or two herds, were fed organically certified feeds. Three of the herds were fed some stored forages at the time of the milk sampling, ranging from 5 to 25 lb/day (as-fed) of corn silage with one of these herds also feeding hay free choice. Grain was fed primarily twice daily, with one herd feeding once and another herd feeding grain three times daily.

Table 1. Characteristics of the 11 herds in the OSU field study.1

Item Mean Range
Number of cows 43 12 to 110
Breed, % of cows for all farms
   Ayrshire 3.6 12 to 110
   Brown Swiss 1.8 12 to 110
   Guernsey 0.5 12 to 110
   Holstein 45.9 12 to 110
   Jersey 23.2 12 to 110
   Mixed 25.0 12 to 110
Milk, lb/day 50.3 4 to 82
Milk fat, % 3.44 2.70 to 4.00
Milk true protein, % 2.98 2.80 to 3.20
Milk urea nitrogen, mg/dl 12.9 10.0 to 17.5
SCC, (cells/ml) x 1000 388 161 to 931
Days on pasture at data collection 53 35 to 70
Type of pasture
   Grass, % 66 35 to 70
   Legume, % 34 35 to 70
Grain DM fed, lb/day2 9.8 0 to 23.4
1One herd fed 5.0 lb/day (as-fed) of corn silage and free choice hay and two herds fed 15 to 25 lb/day (as-fed) of corn silage.
2Assumed concentrates were 90% DM.


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