A. Hilmaz, M. E. Davis1, and H. C. Hines
The Ohio State University Department of Animal Sciences
The objective of this study was to search for mutations in the ovine IGF-I gene that may be related to production traits such as live weight and number of multiple births using Polymerase Chain Reaction (PCR) and Single Strand Conformation Polymorphism (SSCP). A fragment of approximately 265 bp in the 5’ flanking region of the ovine IGF-I gene, about 530 to 795 bp upstream from the 3’ end of exon 1, was amplified for SSCP analysis. Sequences of primers that were used in PCR were 5’-ATTACAAAGCTGCCTGCCCC-3’ (forward primer) and 5’-CACATCTGCTAATACACCTTACCCG-3’ (reverse primer). A PCR-SSCP polymorphism was detected in the 5’ flanking region of the ovine IGF-I gene. Relationships of the polymorphism with production traits in sheep will be investigated.
1 For more information, contact at: The Ohio State University, 221 Plumb Hall, 2027 Coffey Road, Columbus, OH 43210, 614-688-4984, Fax 614-292-2929, e-mail: davis.28@osu.edu